Journal: Cell

Article Title: Integrin Mechano-chemical Signaling Generates Plasma Membrane Nanodomains that Promote Cell Spreading

doi: 10.1016/j.cell.2019.04.037

Figure Lengend Snippet: Key Resources Table

Article Snippet: For estimating GPI-AP anisotropy within regions of the membrane that spatially correlated with vinculin clusters ( and ), the vinculin clusters were first segmented using the blobSegmentThreshold.m function (Danuser laboratory, UT Southwestern Medical Center, USA) along with a gradient filter, and these masks were used to extract intensities from the GPI-AP I pa and Gfactor corrected I pe images using the region properties toolbox in MATLAB (Mathworks,USA).

Techniques: Purification, Transduction, Recombinant, Clinical Proteomics, Avidin-Biotin Assay, Membrane, Transfection, Plasmid Preparation, Stable Transfection, Expressing, Mutagenesis, Derivative Assay, Control, Construct, shRNA, Software